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KMID : 1098420220300030187
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Korean Journal of Medicinal Crop Science
2022 Volume.30 No. 3 p.187 ~ p.194
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Seed Morphology and Germination Characteristics of Rehmannia glutinosa
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Han Jong-Won
Kim Ei-Hyun
Oh Myeong-Won
Lee Jeong-Hoon
Ma Kyung-Ho
Yoon Young-Ho
Jeong Jin-Tae
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Abstract
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Background: This study was conducted to determine the effect of temperature, light, hormones, and after-ripening treatment on Rehmannia glutinosa seed germination to provide valuable infor?mation for its breeding and cultivation.
Methods and Results: The seeds used in this study were harvested from the ‘Jihwang-1-ho’ culti?var. The seed length and width were 1.13 ± 0.03 ㎜ and 0.86 ± 0.02 ㎜, respectively, with a seed coat characterized by polygonal cells. The germination efficiency was evaluated at various tem?peratures (15, 20, 25, and 30℃) and light conditions (light/dark). The optimal germination condi?tion was found to be 25℃ with light (91% germination). Pre-soaking the seeds with gibberellic acid (GA3) increased germination, and the highest germination (72%) was observed with 1,000 ㎎/ ℓ GA3. Additionally, this also increased the mean germination time , germination uniformity, and germination index. The seeds that matured at 45% and 70% relative humidity showed the highest germination rates at 32 and 24 weeks, respectively.
Conclusions: The best method for R. glutinosa seed germination is maintaining 25℃ under light conditions with after-ripening treatment for over 16 weeks. Pre-soaking the seeds in 1,000 ㎎/ℓ GA3 for 24 h further improved their germination. Rehmannia glutinosa seeds were classified under non-deep physiological dormancy.
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KEYWORD
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Rehmannia glutinosa, Seed, Morphological Characteristics, Germination, Dormancy
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